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KMID : 1143120190090040032
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Asia Pacific Allergy
2019 Volume.9 No. 4 p.32 ~ p.32
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Respiratory syncytial virus increases eosinophil extracellular traps in a murine model of asthma
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Silveira Josiane Silva
Antunes Gessica Luana
Gassen Rodrigo Benedetti
Breda Ricardo Vaz
Stein Renato Tetelbom
Pitrez Paulo Marcio
da Cunha Aline Andrea
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Abstract
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Background: Respiratory viral infections are the leading cause of asthma exacerbations. Eosinophil activation results in the formation of eosinophil extracellular traps (EETs), which release web-like structures of DNA and proteins that bind, disarm and extracellularly kill pathogens.
Objective: We investigated whether the respiratory syncytial virus (RSV) in vitro could induce EETs in bronchoalveolar lavage fluid eosinophils in a murine model of asthma.
Methods: BALB/cJ mice (6?8 weeks old) were sensitized with 2 subcutaneous injections of ovalbumin (20 μg) on days 0 and 7, followed by three intranasal challenges with ovalbumin (100 μg) on days 14, 15, and 16 of the protocol. The control group received Dulbecco's phosphate-buffered saline. Bronchoalveolar lavage fluid eosinophils of ovalbumin group or control group were stimulated with RSV (103 PFU/mL) in vitro for 3 hours. After that, culture supernatant was collected to perform the analyses proposed in this study.
Results: We verified an increase in extracellular DNA concentration in bronchoalveolar lavage fluid eosinophils from ovalbumin group stimulated with RSV (103 PFU/mL) in vitro, which was confirmed by confocal microscopy. We demonstrated that most cells are negative for annexin V and propidium iodide in all groups evaluated. Also, RSV in vitro decreased interferon-? in culture supernatant when compared to the ovalbumin group.
Conclusion: In this study, we demonstrated for the first time that RSV in vitro induces EETs formation in eosinophils from asthmatic mice.
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KEYWORD
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Asthma, Inflammation, Eosinophil peroxidase, Extracellular traps, Respiratory syncytial viruses
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